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生物科技 : 学术学位
Expression of alpha-N-acetylglucosaminidase fused to the HIV-1 protein transduction domain and a modified protein transduction domain.
The genetic disorder mucopolysaccharidosis IIIB, which primarily affects the central nervous system (CNS), is caused by a deficiency in the enzyme alpha-N-acetylglucosaminidase (Naglu). Recombinant Naglu is unable to enter cells or cross the blood-brain barrier (BBB), making potential enzyme replace
The popularity of sport amongst all sections of society is a result of a complex interaction with those that it touches. It is also revealing of a malleability which has always enabled people to mould sport as they saw fit and glean from it what they wanted. This thesis aims to articulate this inter
For centuries, patents have served as an economic incentive for promoting research and development by conferring time-limited exclusive rights. The history of biotechnology patents, and more specifically, patents related to genetics is relatively recent. Both case law and legislation have shaped the
The Ackley high-silica magmatic/metallogenic system and associated post-tectonic granites, southeast Newfoundland.
The Ackley Granite Suite occupies 2,415 km;Much of the Ackley Granite Suite exhibits geochemical signatures of A-type granites, and a muscovite-bearing phase occurs in the north. Geochemical enrichment/depletion trends culminate at significant, endocontact, spatially separate, aplite-pegmatite Mo de
Comparison of wind stress algorithms and their influence on the wind stress curl using buoy measurements over the shelf of Bodega Bay, California.
In many cases, difficulties in obtaining the wind stress data cause the wind stress to be computed from bulk formulas that estimate the turbulent fluxes on the basis of standard meteorological data. Since this approach to the wind stress evaluation is used in climate and ocean modeling, the goal of
A study was undertaken to investigate the adsorption of two proteins, bovine serum albumin (BSA) and pepsin, to a gold surface using surface plasmon resonance (SPR). The SPR experiment was constructed for the study of fluid samples and the performance of the experiment was evaluated using custom dat
This thesis examines the role of diagnosis---traditional and molecular---in three areas of medicine: personalized cancer treatment, treatment of infectious diseases and treatment of controversial disorders lacking unambiguous physiological bases. The thesis uses a mix of statistics, expert interview
Worldwide phylogeny of the damselfly genus Ischnura based on mitochondrial cytochrome oxidase II and cytochrome B sequence data.
The first molecular phylogeny for North American members of the genus Ischnura was presented by Chippindale et al. (1999). Here, this work has been expanded in two ways. A second individual of each North American species (when possible) has been included, and the scope of the analysis includes memb
Communities of fungi associated with roots and nodules of Alnus incana subsp. tenuifolia and the roots of Betula papyrifera collected at four sites in the aspen parkland ecosystem around Edmonton, Alberta, were described using taxonomic and physiological approaches. Patterns in community structure,
The duplication of genes is a source of new genes, and thus can be of great interest. This thesis presents a simple model of gene duplication and gene loss, and a method by which the probability of a gene phylogeny can be computed for particular rates of these processes. This allows the likelihood o
This dissertation focuses on the empirical generalizations related to retailer promotions. Two essays are developed addressing the generalizations. The first essay addresses the contradiction in the calculations of primary and secondary demand effects. A mathematical proof is developed to show that
Improving quantification and detection in PET imaging: Methods for image generation, analysis, and consequential clinical considerations.
The field of PET imaging has been continually evolving towards better imaging capabilities, and improved clinical diagnosis. At the intersection of several specialties: physics, mathematics, computer science, and medicine, PET imaging has abilities, bases and limitations distributed abound. In this
Characterization of porcine reproductive and respiratory syndrome virus replication in laboratory rodents.
Porcine reproductive and respiratory syndrome virus (PRRSV) is responsible for major economic losses to the swine industry worldwide. Currently, there is no small animal model system for the study of PRRSV. The aim of this study was to examine BALB/c mice, SCID mice, and cotton rats for PRRSV pathog
In order to assess the nutrient limitation of plants and soil microorganisms in the Arctic coastal salt marsh at La Perouse Bay, Manitoba, a nitrogen (N) and phosphorus (P) fertilization experiment was conducted. The partitioning of carbon (C), N and P was measured from June 2003 to July 2004. Soil
Fungal assemblages in mats of Cladonia mitis, C. rangiferina, and Pleurozium schreberi in Manitoba were isolated and compared in July and September. Temporal changes in these fungal assemblages were seen over the two sampling periods. The fungal assemblages of C. mitis and C. rangiferina were signif
A study of orchids was carried out in a cloud forest in southeastern Peru, in the department of Cusco, between September 2005 and August 2006. A systematic collection found 239 different morphospecies of orchids which was compared to those found at two similar areas, Manu National Park and Machu Pic
Lipid bilayers supported on micron-sized spherical polyHEMA beads were prepared. The lipobeads coatings consisted of 100% DMPC, DMPC/cholesterol (70/30) or DMPC/DMPG (1:1). Coating of the beads was established by fluorescence microscopy, of a fluorophore-labeled lipid. The thermotropic phase behavio
The frontal ganglion (FG) is an important regulator of feeding behavior in insects. Three large neurons in the FG of the tobacco hornworm, Manduca sexta, contain nitric oxide synthase (NOS), the enzyme that synthesizes nitric oxide (NO). This small molecule has been implicated as a neurotransmitter
Trap, beam trawl and fishing surveys revealed 58 different species, 18 of which were crustaceans. Hemigrapsus sanguineus, the invasive Asian shore crab, was present in the trap and beam trawl surveys. During the trap survey H. sanguineus accounted for 20.8% of all crabs caught. Results show that the
The effects of temperature and moisture on survivorship, growth, and multilocus heterozygosity-fitness relationships in the earthworm Eisenia fetida (Oligochaeta).
Positive relationships between multilocus heterozygosity (MLH) and fitness have been found in a variety of organisms. This study examined the effects of temperature and soil moisture on survivorship, growth, MLH-survivorship relationships, and MLH-growth relationships in the earthworm Eisenia fetida